- Footage created by Industrial Light and Magic for Star Trek: First Contact showing the launch of Cochrane's ship, the Phoenix, is included in the opening credits.
- This series did not use the traditional sound stages reserved for Star Trek series, because one of the stages, which last held the Star Trek: Voyager sets, was demolished following filming of the last Voyager episode.
- Because the show is 100 years before Kirk, some old technology has reappeared: - flip-open communicators - manual sliders on the transporter - the science station viewfinder - Most of the sound effects for the Enterprise come from original 1960s Star Trek: The Original Series series - including all the bridge sounds, doors, communicator chirps, and most of the panel sounds. - Instead of shields, the Enterprise has polarized hull plating and instead of hand-held phasers, the crew are introduced to phase pistols. - There are no photon torpedoes, simply torpedoes. (Until the start of season 3) - The transporter has only recently been approved for transporting bio-matter (people)...and no one on the crew trusts it. It has 4 docking doors for shuttlepods. The design of 22nd century Enterprise NX-01 bears a striking resemblance to the 24th century Akira Class starship, first introduced in Star Trek: First Contact. Enterprise carries a designation of NX-01 which, according to established canon, indicates a prototype starship. It also indicates the first Starfleet starship to use this naming convention. Enterprise is the first Starfleet vessel to use the new warp 5 engine developed by Zefram Cochrane and Jonathan Archer's father, Henry.
- As with other Star Trek series, the number 47 appears numerous times throughout the show.
- Majel Barrett, Joseph Ruskin, Clint Howard and Jack Donner are the only actors to appear in both this series and the original Star Trek: The Original Series.
- This was the first Star Trek series to have an actual theme song, a fact that caused controversy among fans, who were split on the idea. The pilot episode used a different piece of music for the closing credits (in fact, an instrumental of the opening song) from the rest of the episodes. Beginning with the third season, the theme was revised to be more upbeat.
- Jeffrey Combs (Shran/Krem), Gary Graham (Vulcan Ambassador Soval) and Matt Winston (Crewman Daniels) are the only actors, besides the regulars, to appear in all four seasons.
- The first Star Trek series to not use "starring" and "also starring" in the credits; also the first to not name the characters being played by the actors (i.e. [Actor] as [Character]).
- The solar system in the title sequence is described in German.
- This is the only "Star Trek" series to have the same regular cast throughout its entire run.
- Longtime Trek actor Vaughn Armstrong (famous for his many alien roles on various Trek series) appears here as a human for the first time. His character, Admiral Maxwell Forrest, is the commander-in-chief of the fledgling Starfleet.
- Admiral Forest is named after DeForest Kelley, the late Star Trek: The Original Series actor who played Leonard H. McCoy. Similarly, Commander Williams and Admiral Leonard from the pilot "Enterprise" (2001) {Broken Bow: Part 1 (#1.1)} are named after series stars William Shatner (James T. Kirk) and Leonard Nimoy (Spock).
- The first name of Captain Archer was initially to have been Jeffrey. While the (American) producers of the show didn't see any problems with this name, UK fans pointed out the link to disgraced author, actor and politician Jeffrey Archer after learning of the name over the Internet. The name was changed to Jackson, but there was exactly one person in the country named Jackson Archer. To avoid lawsuits, Jonathan was chosen for a name because there were 20 Jonathan Archers.
- Scott Bakula jokingly suggested prior to the filming of the two-hour premiere that Captain Jonathan Archer's middle name might be Beckett, a reference to his previous television series, Quantum Leap, in which his character was Dr. Sam Beckett.
- It had been reported that NBC, the network that carried the original Star Trek: The Original Series, was interested in acquiring this series, but lost out to UPN.
- The interiors were inspired by a visit to a docked nuclear submarine.
- The bridge set has 80 plasma screens built into it.
- Dominic Keating was denied a callback for a guest shot on Star Trek: Voyager because Rick Berman wanted him for this.
- Travis Mayweather was originally going to be a lieutenant because of his extensive space experience, but was made an ensign due to the age disparity between him and Malcolm Reed.
- Connor Trinneer discovered a lot of his friends were closet Trekkies when he got his role.
- Based on the official Star Trek Chronology, the series begins 10 years prior to the founding of the United Federation of Planets and 90 years after the events of Star Trek: First Contact. Episode one takes place approximately 115 years prior to the start of Star Trek: The Original Series and 213 years before Star Trek: The Next Generation.
- In the Star Trek: The Next Generation episode "Star Trek: The Next Generation" (1987) {Yesterday's Enterprise (#3.15)}, two references are made to planet Archer 4. Since no one has ever mentioned this in connection with Star Trek: Enterprise, it is probably coincidental. In Star Trek: Nemesis, we see a a starship named "Archer" on the tactical display; a planet named after one of the founding fathers of the Federation is not to hard to believe.
- In the wake of the destruction of the US Space Shuttle Columbia on 1 February 2003, an opening screen was added to the episode "Enterprise" (2001) {Stigma (#2.14)} (first air date 5 February 2003), which read "In memory of the Columbia crew...You will always be an inspiration." Further, just as the original US Space Shuttle prototype (which never reached space) was named "Enterprise" after the ship in the original Star Trek: The Original Series, so it was revealed in "Enterprise" (2001) {The Expanse (#2.26)} (first air date 21 May 2003) that the second Starfleet prototype ship (designation NX-02) was named in honor of the "second" Space Shuttle (actually the first to reach space), Columbia. Although the initial glimpses of NX-02 were of an incomplete ship in dry dock, she was seen more extensively in a story arc in season 4. It should also be noted that an "SS Columbia" was mentioned in the original "Star Trek" pilot, "Star Trek" (1966) {The Cage (#1.0)}, and a scout ship "USS Columbia" (designation NCC-621) appears briefly in Star Trek: The Motion Picture.
- During filming of the pilot, Star Trek: The Next Generation actors Brent Spiner (Lt. Commander Data) and Jonathan Frakes (Commander William T. Riker) visited Scott Bakula on the set to give him advice about what to expect while working on the series. Both Brent Spiner and Jonathan Frakes guest starred in season 4 with Brent Spiner playing Data's creator's grandfather Dr. Arik Soong and Jonathan Frakes playing his original character.
- "Star Trek" was originally left off the title because of its overuse in previous franchise titles and because "Enterprise" was just as instantly recognizable, from "Enterprise" (2001) {Broken Bow: Part 1 (#1.1)} all the way through "Enterprise" (2001) {Anomaly (#3.2)}. After the second season suffered low ratings, the third season adopted the title "Star Trek: Enterprise" starting with "Enterprise" (2001) {Extinction (#3.3)}. When "Enterprise" (2001) {The Xindi (#3.1)} re-aired, "Star Trek" was added to the title. However, in re-airing "Anomaly", the title remained simply "Enterprise".
- Enterprise is the first "Star Trek" series to be shot in widescreen format, to allow for HDTV format airing. Enterprise is the fourth Star Trek series in the franchise's history to have a September premiere date, and the first Star Trek series in fifteen years to premiere in September. The original Star Trek: The Original Series, "Star Trek: The Animated Adventures" (1973)_ and Star Trek: The Next Generation all premiered in September. Star Trek: Deep Space Nine and Star Trek: Voyager both premiered in January.
- Originally Jolene Blalock's character of T'Pol was supposed to be called T'Pau. This was the name of a character that appears in "Star Trek" (1966) {Amok Time (#2.1)}, an episode of Star Trek: The Original Series. They changed it at the last minute because if they used the character of T'Pau, they would have to pay royalties to the writer of the original Star Trek episode that she appeared in every time T'Pau was used, i.e. every episode of "Enterprise." Incidentally, the character of T'Pau later made an appearance in the three-part Vulcan renaissance story arc in season 4.
- The Chief Medical Officer of the Enterprise is Doctor Phlox. The name Phlox was a character of the Hierarchy race from the Star Trek: Voyager episode "Star Trek: Voyager" (1995) {Tinker Tenor Doctor Spy (#6.4)}. However, the name of the character in the Voyager episode was only used in the script and was never mentioned on screen.
- According to Jolene Blalock, a decision was made to change T'Pol's make-up - particularly her hairstyle - after three days of shooting. All Blalock's scenes for the first few days had to be reshot.
- Commander Tucker's nickname was originally to be "Spike". His eventual nickname 'Trip' comes from his being the third generation man of his family to be called Charles. Trip is short for triple.

