The Road to El Dorado Trivia
- A series of sequels featuring Miguel, Tulio, Chel, Altivo and even the armadillo going after other legends about gold was planned, similar to the Shrek series, but following the disappointing box-office results they were immediately canceled.
- The name of the Spanish general in the movie is Cortes, but in real life the man who tried to find El Dorado was Gonzalo Pizarro.
- The armadillo that follows Miguel and Tulio throughout is named Bibo, after co-director Bibo Bergeron.
- Tzekel-Kan's sacred book contains a picture of a man fishing from the moon, a parody of the Dreamworks logo.
- Voice artists in animated movies usually record their parts alone, with no other actors in the studio with them. In a break with this tradition, Kevin Kline and Kenneth Branagh recorded their dialogue together.